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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Biochim Biophys Acta. 2009 May 3;1793(7):1272–1278. doi: 10.1016/j.bbamcr.2009.04.013

Fig. 5.

Fig. 5.

Mature LOX appears only in conditioned media. Conditioned media from NIH 3T3 cells was blotted and probed with anti-LOX2527 and anti-LOpep3. Lane 1, NIH 3T3 cell extract; lane 2, 30 μl of NIH 3T3 conditioned media, concentrated from 0.1 ml on Centricon YM-10 column. The strongly hybridizing band at ~55 kDa in lane 2 is immunoglobulin derived from the fetal bovine serum added to cell culture media.