TABLE 3.

Quantitative RNA dot blot analysis of region 1 and 3 transcription

Strain	Band intensitya with probe:
	kpsE		kpsT
	37°C	20°C	37°C	20°C
MS150	100 ± 5	19 ± 2	100 ± 5	17 ± 2
MS152 (hns::kan)	52 ± 10	52 ± 4	45 ± 10	38 ± 4
MS153 (bipA::Tn5)	20 ± 8	49 ± 6	26 ± 6	45 ± 6

^aThe signals were quantified as described in Materials and Methods and are expressed as a percentage of the most intense signal (MS150 at 37°C) for each probe used. The experiment was repeated three times, and results are expressed ± the SEM. The region 1 (kpsE) and region 3 (kpsT)-specific probes were used to hybridize to RNA extracted from cells grown at either 37 or 20°C.