FIG. 1.

Quantitative analysis of INO4 and INO1 mRNAs. (A) Representative Northern blot hybridization showing INO4 transcript levels. TCM1, a constitutively expressed ribosomal protein gene, was used to normalize for loading variations. The relevant genotypes of the strains used are shown in parentheses. (B) Bar values represent ratios of INO4 to TCM1 counts per minute quantified using a Betascope 603 Blot Analyzer (Beta-gen). (C) Bar values represent ratios of INO1 to TCM1 counts per minute. Cells were grown in complete synthetic medium supplemented with 75 μM inositol and 1 mM choline (I+75C+) or 10 μM inositol and 1 mM choline (I+10C+) or lacking inositol and choline (I−C−). The values shown are averages of at least three separate trials and are normalized to that of the wild type without inositol and choline (100%).