FIG. 2.

Expression of INO4-cat fusions in the wild type (WT) (BRS1001), ino4Δ (BRS2004), and ino2Δ (BRS2001) strains. Transformants containing the various promoter deletions were grown in uracil-lacking synthetic medium either containing 75 μM inositol and 1 mM choline (I+C+) or lacking inositol and choline (I−C−). Extracts from the yeast transformants were prepared and assayed for CAT activity. The values shown are averages of at least three separate trials. YOL107W is a divergent ORF. The locations of two putative bHLH binding sites and a potential TATA box are shown. N.D., not determined.