Buffer	Description	Recipe
1 × lysis buffer, 10 mL	20mM Tris pH 7.9, 500mM NaCl, 100μM PMSF, 10mM imidazole	5mL 2 × lysis buffer 10μL 100mM PMSF 50μL 2M imidazole Adjust to 10mL with H2O
1 × wash buffer, 10 mL	20mM Tris pH 7.9, 500mM NaCl, 100μM PMSF, 25mM imidazole	5mL 2 × lysis buffer 10μL 100mM PMSF 125μL 2M imidazole Adjust to 10mL with H2O
1 × dilution buffer, 10 mL	20mM Tris pH 7.9, 500mM NaCl, 100μM PMSF, 350 mM imidazole	5mL 2 × lysis buffer 10μL 100mM PMSF 1.75 mL 2M imidazole Adjust to 10mL with H2O
1 × TGT storage buffer, 500 mL	25mM HEPES, pH 7.3, 2 mM DTT, 1 mM EDTA, 100μM PMSF	50mL 10 × TGT storage buffer 500μL 100 mM PMSF Adjust to 500 mL with H2O