Skip to main content
. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Methods Enzymol. 2020 Apr 20;641:373–399. doi: 10.1016/bs.mie.2020.03.009

Table 1.

Kinetics values of select RNA substrates as reported by Curnow and Garcia (1995).

Substrate name (Curnow & Garcia, 1995) Stem loop sequence Km, μM Vmax, μMs−1 mg−1 Vmax/Km, s−1 mg−1
ECY2 (unmodified tRNATyr) …GCAGACUGUAAAUCUGC… 2.0 3.0 1.5
ECY-A1 GCAGACUGUAAAUCUGC 13.3 0.48 0.04
ECYMH GGGAGCAGACUGUAAAUCUGCUCCC 6.9 2.2 0.3
SCDMH GGCGGCGCUUGUCGCGUGCCGCC 11.4 2.7 0.24
SCDMH-U33C GGCGGCGCUCGUCGCGUGCCGCC 22.4 0.06 0.003
SCDMH-G34A GGCGGCGCUUAUCGCGUGCCGCC N.D. N.D. N.D.
SCDMH-U35C GGCGGCGCUUGCCGCGUGCCGCC N.D. N.D. N.D.

N.D. = no detectable activity.