Table 2.

Kinetic parameters of various nucleobase substrates.

Compound	Km, μM	kcata (×10−3) s−1	kcat/Kma (×10−3) s−1 μM−1	Ref.
Guanine	2.2	61a	28 a	Hoops et al. (1995)
−CH2NH2 (preQ1)	0.39	45a	115a	Hoops et al. (1995)
−CN (preQ0)	2.35	72a	31 a	Hoops et al. (1995)
−CH2OH	23.0	45a	2.0a	Hoops et al. (1995)
−H	172	45a	0.3a	Hoops et al. (1995)
−CH3	255	45a	0.2a	Hoops et al. (1995)
−CH2OCH3	57	47a	0.8a	Hoops et al. (1995)
−CH2N(CH3)2	75	43a	0.6a	Hoops et al. (1995)
−CONH2	26	69a	2.7a	Hoops et al. (1995)
−CO2CH3	87	73a	0.8a	Hoops et al. (1995)
−COCH3	26	73a	2.8a	Hoops et al. (1995)
−CHO	22	73a	3.3a	Hoops et al. (1995)
−CO2H	126	21a	0.2a	Hoops et al. (1995)
−(PEG)3-thiazole orange	9.8	1.6	0.2	Alexander et al. (2015)
−C6H12-thiazole orange derivative	1.6	26.7	16.7	Zhou et al. (2017)

^ak_cat were calculated from reported V_max values using the reported enzyme concentration (50 nM), and reaction volume (400 μL) (Hoops et al., 1995).