| Reagent | Final concentration |
|---|---|
| 10 × TGT reaction buffer | 1 × (100 mM HEPES, pH 7.3, 20 mM MgCl2) |
| 100mM DTT | 5mM |
| RNA transcript | 1μM |
| TGT enzyme | 1μM |
| preQ1-C6H12-derivative | 10μM |
| RNAse inhibitor, murine | 1U/μL |
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