FIG. 5.

Time course of 2-hydroxychc-CoA dehydrogenase activity. Activity was measured spectrophotometrically by monitoring the reduction of NAD⁺ at 340 nm using standard assay conditions as described in Materials and Methods. Reaction mixtures contained purified His-BadH (0.8 μg) plus purified 2-ketocyclohexanecarboxyl-CoA hydrolase (10 μg) (triangles), hydrazine (60 mM) (squares), or neither hydrazine nor 2-ketocyclohexanecarboxyl-CoA hydrolase (circles).