FIG. 6.

Time course of anaerobic benzoate transformation by cultures of CGA702 (badH::Km^r). Cells were grown anaerobically in mineral medium containing 10 mM succinate and 1.5 mM benzoate. Aliquots were removed at various time points, cells were removed by centrifugation, and supernatants were analyzed by C₁₈ reverse-phase HPLC for the presence of benzoate (squares) and cyclohex-1-ene-carboxylate (circles). Growth (represented by triangles) was monitored spectrophotometrically by monitoring optical density at 660 nm (OD_{660 nm}). Data points are averages of duplicates.