FIG. 1.

Construction of overexpression plasmids for RP4 trbB, cag HP0525, and R388 trwD. Upper panels, nucleotide sequences flanking the initiation codons of trbB (A), HP0525 (B), and trwD (C) in their native environments. Nucleotide sequences resembling Shine-Dalgarno sequences (“S/D”) are underlined. Initiation codons are marked by black boxes. Partial amino acid sequences of gene products are shown below the nucleotide sequences. Lower panels, genes were placed under translational control by the T7 gene 10 ribosomal binding site (S/D) by insertion into the expression vector pMS470Δ8. Underlined parts of the amino acid sequences were confirmed by N-terminal sequencing.