TABLE 2.

Purification of TrbB, HP0525, and TrwD

Protein	Fraction	Purification step	Protein (mg)	Yield (%)	Purity (%)
TrbB	I	Crude cell extract	912	100	27.1
	II	(NH4)2SO4 precipitation	600	65.8	32.3
	III	Heparin-Sepharose CL-6B chromatography	262	28.7	47.6
	IV	Hydroxylapatite chromatography	85	9.3	99.8
HP0525	I	(NH4)2SO4 precipitation	918	100	10.7
	II	HiTrap Q chromatography	146	38	25.6
	III	Hydroxylapatite chromatography	19	17	89.7
	IV	Superose 12 gel filtration	6.1	6	98.3
TrwD	I	Crude cell extract	1075	100	31.9
	II	(NH4)2SO4 precipitation	280	26.1	32.5
	III	Heparin-Sepharose CL-6B chromatography	135	12.6	35.4
	IV	Hydroxylapatite chromatography	23	2.1	93.4