TABLE 1.
Comparison of common CRISPR systems.
| Effector | Domain | Target type | Signal RNA | Detection platform | Ampli-fication | Quanti-tative | Time | Sensitivity | Portable |
|---|---|---|---|---|---|---|---|---|---|
| Cas9 | HNH, RuvC | DNA | tracrRNA, crRNA | - | CAS-EXPAR | No | <1 h | aM | Yes |
| Cas12a | RuvC | DNA | crRNA | HOLMES, DETECTR | HOLMES: PCR; DETECTR: RT-PCR, RPA | No | 1 h | aM | HOLMES: yes; DETECTR: no |
| Cas12b | RuvC | DNA | crRNA | HOLMESv2 | LAMP, RT-LAMP | Yes | 1 h | aM | Yes |
| Cas13a | HEPN | RNA | crRNA | SHERLOCK | RPA | No | 2 h | aM | Yes |
| Cas14 | RuvC | ssDNA | tracrRNA, crRNA | DETECTR | RPA | No | 1 h | aM | Yes |
RPA, recombinase polymerase amplification.