Fig 2. CMV DNAemia specific protein signatures exist pre-CMV DNAemia timepoint and post-CMV.

[A] Plasma protein profiles for pre- and post-CMV DNAemia samples CMV DNAemia positive and negative cohort were analyzed using linear discriminant analysis (LDA). LDA was able to separate samples based on CMV status among CMV DNAemia positive and CMV DNAemia negative cohort. Within CMV DNAemia-positive cohort the samples were separated based on post-CMV infection time. Among samples collected from CMV DNAemia-positive individuals, samples collected 1-week and 1-month post-infection were interspersed suggesting the signal of CMV infection persists until 1-month post-infection. [B] Plasma samples were collected at pre-CMV infection time from CMV DNAemia positive and matching CMV DNAemia negative cohort. The analysis resulted in a set of 17 proteins whose levels were either increased (n = 6) or decreased (n = 11). The significance of the changes is demonstrated by a Volcano plot. The most significantly increased protein was Lysine Methyltransferase 2C (KMT2C) with 3.38 fold increase (p = 0.05) in CMV DNAemia positive samples and most significantly decreased protein was Immunoglobulin Lambda Variable 7–43 (IGLV7-43) with 2.17 fold decrease (p = 0.01). [C] We analyzed samples collected at 1-week and 1-month post-CMV infection from CMV DNAemia-positive cohort and compared them against proteins profiles generated from CMV DNAemia-negative cohort that included baseline and 1-year time-point. This analysis resulted in significant changes in 16 proteins, with an increase in their level with CMV infection (n = 11) and a decrease in their level with CMV DNAemia (n = 5). Changes in individual proteins are presented as a volcano plot.