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. 2023 May 9;21(5):e3002111. doi: 10.1371/journal.pbio.3002111

Fig 6. Expression of chemokine scavengers in small intestine and colon.

Fig 6

Confocal imaging of small intestine from (A) ACKR3GFP/+ − GPR182mCherry/+ mouse, GPR182 (mCherry, red), ACKR3 (GFP, green), CD31 (pink), and Lyve-1 (cyan) (scale bar = 70 μm), and (B) ACKR4GFP/+ − GPR182mCherry/+ mouse, GPR182 (mCherry, red), ACKR4 (GFP, green), CD31 (pink), and Lyve-1 (cyan) (scale bar = 100 μm). Arrows point to central lacteal (GPR182+, mCherry, red) and submucosal lymphatics (left and right). (C) Immunofluorescence image of a section from small intestine from an ACKR4GFP/GFP mouse, showing ACKR4 expressing cells (GFP, green). Smooth muscle cells are positive for α-SMA (red), and cell nuclei in DAPI (blue), scale bar: 20 μm. (D) Immunofluorescence image of a section from small intestine from an ACKR4GFP/GFP mouse, showing ACKR4 expressing cells (GFP, green) in close proximity to c-kit–positive Cajal cells (red); cell nuclei DAPI (blue), scale bar: 20 μm. (E) Confocal images of colon from an ACKR3GFP/+ − GPR182mCherry/+ mouse, GPR182 (mCherry, red), ACKR3 (GFP, green), CD31 (pink), and Lyve-1 (cyan) (scale bar = 100 μm). (F) Confocal images of colon from an ACKR4GFP/+ − GPR182mCherry/+ mouse, GPR182 (mCherry, red), ACKR4 (GFP, green), CD31 (pink), and Lyve-1 (cyan); arrows indicate the lymphatic vessels (scale bar = 100 μm). (G) Immunofluorescence image of a section of colon of an ACKR4GFP/GFP mouse, showing cells expressing ACKR4 (GFP, green) and α-SMA (red); cell nuclei DAPI (blue), scale bar: 20 μm. (H) Immunofluorescence image of a colon section from an ACKR4GFP/GFP mouse, showing ACKR4 expressing cells (GFP, green) in close proximity to c-kit–positive Cajal cells (red); cell nuclei DAPI (blue), scale bar: 10 μm. GFP, green fluorescent protein.