Table 1. Cryo-EM data collection, refinement and validation statistics.
| FliF RBM3 (EMDB-29425) (PDB:8FTF) | FliF RBM2 (EMDB-29424) (PDB:8FTE) | |
|---|---|---|
| Data Collection | ||
| Microscope | Thermo Fisher FEI Titan G4 | Thermo Fisher FEI Titan G4 |
| Detector | Falcon-III DED | Falcon-III DED |
| Magnification | 120,000x | 120,000x |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e-/Å2) | 50 | 50 |
| Defocus range (μm) | -0.8 to -2.2 | -0.8 to -2.2 |
| Pixel size (Å) | 0.6811 | 0.6811 |
| Total micrographs taken | 5,597 | 11,627 |
| Data Processing | ||
| Micrographs used for processing | 5,597 | 10,707 |
| Symmetry imposed | C33 | C22 |
| Initial particle images | 336,352 | 173,942 |
| Final particle images | 32,042 | 100,096 |
| Map resolution (Å) | 2.91 | 3.8 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Initial model used (PDB) | PDB: 6SCN–RBM3 | PDB: 6SD5 |
| Model resolution (Å) | 2.25 (masked), 3.0 (unmasked) | 3.7 (masked), 3.8 (unmasked) |
| FSC threshold | 0.143 | 0.143 |
| Model resolution range (Å) | 100–2.25 | 100–3.7 |
| Model composition | ||
| Total atoms | 1285 | 667 |
| Water | 0 | 0 |
| Protein residues | 165 | 93 |
| Bonds (RMSD) | ||
| Bond lengths (Å) (# > 4σ) | 0.002 | 0.003 |
| Bond angles (°)(# > 4σ) | 0.559 | 0.621 |
| Validation | ||
| MolProbity score | 1.14 | 2.06 |
| Clash score | 3.53 | 3.69 |
| Rotamers outliers (%) | 0 | 7.9 |
| Ramachandran plot | ||
| Favored (%) | 98 | 96.63 |
| Allowed (%) | 2 | 3.37 |
| Disallowed (%) | 0 | 0 |