Skip to main content
. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Nanomedicine. 2020 Jun 28;29:102257. doi: 10.1016/j.nano.2020.102257

Figure 2.

Figure 2.

Secretion of cytokines induced by stimulated DCs. DCs were exposed to various concentrations (0.01, 0.1 and 1 μg/mL) of bare rMOMP or PLGA-rMOMP or an equivalent weight of PLGA-PBS. Cell-free supernatants were collected at 24 h, and quantifications of IL-6 (A), IL-12p40 (C), IL-10 (E), and TNF-α (G) were performed using cytokine-specific ELISAs. Cumulative production of cytokines was determined after exposing DCs to 0.01 μg/mL of bare rMOMP or PLGA-rMOMP. Cell-free supernatants were collected at 4, 24, 48, 72, and 96 h time-points to quantify IL-6 (B), IL-12p40 (D), IL-10 (F), and TNF-α (H). Each bar represents the mean ± standard deviation of triplicate samples. Experiments were repeated at least three times. Significance between groups was established at ****P < 0.0001 **P < 0.01 and *P < 0.05.