FIG. 5.

(A) Binding of Rci protein to the four shufflon segments. DNA fragments of pKK019-19 (0.1 pmol) digested with HaeIII and SspI (see Fig. 1A) were incubated with the indicated amounts of Rci protein and electrophoresed on a polyacrylamide gel as described in Materials and Methods. (B) Binding of Rci protein to four 19-bp sequences. The names of the 19-bp sequences are indicated at the top. −, control without a 19-bp sequence. PCR-generated fragments carrying the 19-bp sequences (0.1 pmol) were incubated without (−) or with (+) 6.8 pmol of Rci protein. A binding assay was performed as described in Materials and Methods except that bovine serum albumin was omitted from the reaction mixtures.