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. 2023 May 5;12:e85714. doi: 10.7554/eLife.85714

Figure 7. Targeted overexpression of Hif1a in osteoblasts rescues low bone mass in Type 2 diabetes (T2D) mice.

(A) A schematic for Hif1a overexpression (Hif1OE) in normal or T2D conditions. (B) qPCR analyses of glycolysis-related genes in bones of diabetic mice with or without Hif1a overexpression. n=4. (C–E) Representative µCT images (C) and quantification of cortical bone (D) and distal trabecular bone (E) of the femur. n=10. (F, G) Representative images (F) and quantification (G) of double labeling in trabecular bone of proximal tibia in diabetic mice. Ctrl, n=5, Hif1OE, n=4. Data presented as mean ± SD. *p<0.05 Unpaired Student’s t-test (B, G) or two-way ANOVA followed by Fisher’s LSD test (D, E).

Figure 7.

Figure 7—figure supplement 1. Hif1a overexpression improves bone mass in Type 2 diabetes (T2D) mice.

Figure 7—figure supplement 1.

(A, B) Cortical (A) and trabecular (B) bone parameters by µCT. (C) Mineral apposition rate (MAR) from double labeling in the T2D group. Ctrl, n=5; Hif1OE; n=4. Data are represented as mean ± SD. *p<0.05, two-way ANOVA followed by Fisher’s LSD test (A, B) or Student’s t-test (C).
Figure 7—figure supplement 2. Glut1 overexpression does not improve bone in Type 2 diabetes (T2D) mice.

Figure 7—figure supplement 2.

(A) Glut1 overexpression in the bone shaft by Western blot. (B) Representative µCT images. (C) Quantification by µCT. Ctrl, n=8; Glut1OE; n=9. (D) Serum P1NP and CTX-1 levels. Ctrl, n=6; Glut1OE; n=5. Data are represented as mean ± SD. *p<0.05 by Student’s t-test.
Figure 7—figure supplement 2—source data 1. Raw images of Western blots for Figure 7—figure supplement 2A.
Figure 7—figure supplement 2—source data 2. Raw western blots.