KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-NKCC1	Abcam	ab59791; RRID: AB_944433
Rabbit anti-pNKCC1	EMD Millipore	ABS1004; RRID: N/A
Rabbit anti-GAPDH	Sigma	G9545; RRID: AB_796208
Mouse anti-HA	Abcam	ab130275; RRID: AB_11156884
Rat anti-HA	Roche	11867423001; RRID: AB_390918
Rabbit anti-FLAG	Abcam	ab1162; RRID: AB_298215
Rabbit anti-Iba1	WAKO	019-19741; RRID: AB_839504
Rat anti-CD68	Abcam	ab53444; RRID: AB_869007
Bacterial and Virus Strains
AAV2/5-NKCC1	Modified from Somasekharan et al.54	Addgene plasmid #49077
AAV2/5-NKCC1-NT51	Modified from Somasekharan et al.54	Addgene plasmid #49061
AAV2/5-GFP	Boston Children’s Hospital viral core, IDDRC	N/A
Biological Samples
Human CSF	Massachusetts General Hospital	N/A
Chemicals, Peptides, and Recombinant Proteins
Paraformaldehyde	Sigma	P6148
EGTA	Sigma	E3889
BAPTA-AM	Tocris	2787
2-APB	Tocris	1224
LiCl	Sigma	203637
Clarity™ Western ECL Substrate	BioRad	170-5060
Critical Commercial Assays
Deposited Data
Blood proteomics	ProteomeXchange Consortium via the PRIDE partner repository	PXD039738
Experimental models: Cell lines
Experimental Models: Organisms/Strains
CD-1 IGS Mouse (referred to as CD-1)	Charles River Laboratories	Strain code: 022
FoxJ1-CRE	Zhang et al.76	N/A
Ai95D(RCL-GCaMP6f)-D	Jackson Laboratory	028865; RRID:IMSR_JAX:028865
Oligonucleotides
Recombinant DNA
Software and Algorithms
Prism	GraphPad	v7
FIJI/ImageJ	Schindelin et al.84	http://imagej.net/Fiji;https://imagej.nih.gov
ZEN Black/Blue	Zeiss	N/A
AMIDE	https://amide.sourceforge.net/	N/A
MatLab	Mathworks	R2019b
Other