Fig. 2.

TAMs mediate radioresistance of ESCCs and formation of immunosuppressive TME. a, DNA damage sensitivity of ESCC cells to radiotherapy examined by EdU labeling; b, necrosis of ESCC cells analyzed by Calcein AM and 7-AAD staining; c-d, migration (c) and invasion (d) of ESCCs analyzed by Transwell assays; e, release of immunosuppression marker TGF-β by macrophages analyzed by ELISA kits; f, proliferation of active T cells co-cultured with macrophages analyzed by CFSE staining. Differences of the normally distributed data between groups were analyzed by two-way ANOVA (a–e). Significance of difference was analyzed by Sidak's multiple comparisons test (a–d) or Dunnett's multiple comparisons test (e–f). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001