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. 2023 Jan 23;72(6):1835–1851. doi: 10.1007/s00262-022-03364-5

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The LINC01004-SPI1 axis-mediated SIGLEC9 affects TAM-mediated radioresistance and immunosuppression in ESCC cells. a, phenotype change of TAMs with SPI1 shRNA or LINC01004 ASO transfection analyzed by flow cytometry; b, c, expression of M2 phenotype markers IL-10 and PD-L1 (b) and M1 phenotype markers TNF-α and IL-12 (c) in TAMs with SPI1 shRNA or LINC01004 ASO transfection analyzed by qPCR analysis; d, DNA damage in ESCC cells examined by EdU labeling; e–f, migration (e) and invasion (f) of ESCCs analyzed by Transwell assays; g, necrosis of ESCC cells analyzed by Calcein AM and 7-AAD staining; h, release of immunosuppression marker TGF-β by TAMs analyzed by ELISA kits; i, proliferation of active T cells co-cultured with TAMs analyzed by CFSE staining. Differences of the normally distributed data between groups were analyzed by two-way ANOVA (a–i). Significance of difference was analyzed by Sidak's multiple comparisons test (a–i). **p < 0.01, ***p < 0.001, ****p < 0.0001