Figure 5.

Spheroids spread rapidly in large and small microgel scaffolds. A) DAPI/Phalloidin stains illustrate that void space inherent in microgel scaffolds facilitates rapid spreading in large and small microgel scaffolds. Scale bars represent 200 μm. B) Migration distance did not differ between microgel scaffolds. C) Cell spreading is denser in small microgel scaffolds versus large microgel scaffolds at Day 1 and Day 7. D) alamarBlue assay confirms that both scaffolds promote high cell metabolic activity. Statistics: Two-way ANOVA, Tukey’s multiple comparisons test. n ≥ 3. *p < 0.05, **p < 0.01, and ***p < 0.001