Figure 6.

Microgel size influences macrophage polarization. A) Macrophages (red) in small microgel scaffolds are trapped between them and exhibit an elongated cell morphology (white arrows). Macrophages in large microgel scaffolds are more rounded and can be found in clusters. (B,E) Cell viability is similar in both small and large microgel scaffolds. (C,F) M1 polarization is trending towards being higher in the smaller microgel scaffolds. (D,G) M2 polarization is greater in large microgel scaffolds. Scale bars represent 100 μm. Statistics: Unpaired Student’s t tests. n ≥4. *p < 0.05, **p < 0.01.