FIG. 5.

Disruption of the LIP2 and XPR6 genes. Physical maps of the lipase (LIP2, A) and endoprotease (XPR6, B) genes and disruption cassettes are shown. ORFs are represented by arrow boxes indicating the direction of transcription. (A) For LIP2 gene disruption, the BglII fragments were replaced with a BglII-BamHI LEU2 gene and the NdeI-BamHI linear fragment was used for transformation. (B) For XPR6 gene disruption, the BamHI-HindIII fragment was replaced with a BamHI-HindIII LEU2 gene and the DraI-SalI linear fragment was used for transformation. The sizes of the PstI (A) and NcoI (B) fragments obtained by Southern blotting of the wild-type (WT) and disrupted (KO) strain DNAs using LIP2 (A) and XPR6 (B) probes are shown. Restriction sites: B, BamHI; Bg, BglII; D, DraI; H, HindIII; N, NcoI; Nd, NdeI; P, PstI; S, SalI.