FIG. 6.

Analysis of the extracellular lipase from JMY184 and JMY278 by SDS-PAGE. The equivalent of 5 μl of the supernatant (52 h) from strains JMY184 (LIP2^A; mature form) (Fig. 4B, lane 5) and JMY278 (xpr6 KO; pro form) (Fig. 4C, lane 5) was treated with endo H. Untreated (lanes 1 and 3) and deglycosylated (lanes 2 and 4) proteins were separated by SDS–12% PAGE and stained with Coomassie blue. The positions of endo H and the untreated (Lip2p) and deglycosylated (Lip2p D) lipases are indicated on the left. Prestained molecular mass markers were used as standards (lane 5).