Table 2.

P. falciparum asexual blood-stage minimal inoculum for resistance (MIR) selection summary

Name	Chemical class	Starting selective pressure	Resistant parasites in 4 wells of 2.5 × 106	Resistant parasites in 3 wells of 3 × 107	Resistant parasites in 3 flasks of 3 × 108	MIR	Selected proteasome mutation	IC50 fold shift against selection compound
WLL	vinyl sulfone	3 × IC50 (50.4 nM)	none	none	none	>1 × 109	N/A	N/A
J-71	epoxyketone	3 × IC50 (152.4 nM)	none	1/3 wells	N/D	1 × 108	β5 A50V	3.1×
J-80	epoxyketone	3 × IC50 (88.2 nM)	none	none	3/3 flasks	3 × 108	β5 M45R, β5 M45V	35.1×, 11.6×
TDI-8304	macrocyclic peptide	3 × IC50 (33 nM)	none	3/3 wells	N/D	3 × 107	β6 S157L, β6 N151Y	2,621×, 1.7×

IC₅₀ fold shifts were calculated as the ratio of the IC₅₀ of a compound against the selected mutant over its IC₅₀ against the Dd2-B2 parental line. N, n = 3–20, 2. N/D, not done; N/A, not applicable. MIRs were calculated as the total number of parasites inoculated divided by the total number of positive cultures (when obtained). The formula extends up to the largest inoculum at which parasites were obtained (or tested in the case of negative cultures), and includes lower inocula (see STAR Methods).