Figure 2.

(A) Principal coordinates analysis (PCoA) plot of the Bray Curtis dissimilarities for the ruminal microbiota by diet and sampling day. The PERMANOVA R² and P-value for the effect of diet within each day are included on each plot. The percentage of variation explained by each principal coordinate is indicated on the axes; (B) Box and whisker plot of the Shannon diversity index values for the ruminal microbiota by sampling day and diet. *P < 0.05; **P < 0.01; ***P < 0.001. The box indicates the interquartile range (IQR) (middle 50% of the data), the middle line represents the median value, and the whiskers represents 1.5 times the IQR; Control refers to the group of heifers that received DDGS in their diet (n = 15) and Hemp refers to the group that received the hempseed cake inclusion diet (n = 16).