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. 2023 May 19;6:544. doi: 10.1038/s42003-023-04893-0

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, d, g, j, m, p Distribution of cell type-specific markers in intact P21 mouse spinal cord. b, e, h, k, n, q Images of NPC grafts at 4 weeks post-transplantation into sites of cervical SCI. Images within the same column in rows b and e, as well as rows n and q, are different fluorescent channels of the same tissue section. c, f, i, l, o, r Quantification of the abundance of c Tlx3⁺, f Lbx1⁺, i clustered calbindin⁺, l Brn3a⁺, o Foxp2⁺, and r Chx10⁺ interneurons in NPC grafts. e Note the different populations of clustered calbindin⁺ neurons (arrow) and non-clustered, larger calbindin⁺ neurons (arrowheads). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons test. E11.5 (n = 11); E12.5 (n = 16); E13.5 (n = 16); dorsal (n = 12); ventral (n = 8). All data are mean ± SEM. Scale bars = 250 μm (a, d, g, j, m, p); 100 μm (b, e, h, k, n, q). Source data are provided as a Source Data file. The experiments in panels a, b, d, e, g, h, j, k, m, n, p, and q were performed twice with similar results.