Fig. 2.

Cytotoxicity, distribution and uptake of AgNPs in HT22 cells. (A–B) Cytotoxicity of HT22 cells, after AgNPs (0–12 μg/mL) or AgNO₃ (0–1.2 μg/mL) exposure for 24 h; (C) Cellular distribution of AgNPs in HT22 cells, after AgNPs (8 μg/mL) exposure for 24 h, the red arrow indicates engulfed AgNPs; (D–F) Silver content (D), Intracellular Ag⁺ content (E) and released Ag⁺ content in cell culture medium (F) in HT22 cells, after AgNPs (0–8 μg/mL) exposure for 24 h; (G–H) Dose-dependent scattering intensities as a percentage of control for HT22 cells treated with AgNPs (0–8 μg/mL) or Ag⁺ (0–0.6 μg/mL) for 24 h. NPs: nanoparticles; Mt: mitochondria. Data are expressed as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 when compared with the control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)