Fig. 6.

ROS promoted mitochondrial fragmentation and mitochondrial dysfunction induced by AgNPs in HT22 cells. HT22 cells were pretreated with/without NAC (5 mM) 2 h before exposure to 8 μg/mL AgNPs for 24 h. (A) Alteration of Mitotracker fluorescence in HT22 cells; (B–C) Intracellular ROS levels in HT22 cells; (D–E) MMP changes in HT22 cells; (F) ATP content in HT22 cells. Data are expressed as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 when compared with the control. ^#p < 0.05, ^##p < 0.01 when compared with the AgNPs (8 μg/mL) group.