Fig. 7.

ROS promoted mitochondrial fission/fusion and enhance mitochondria-dependent apoptosis induced by AgNPs in HT22 cells. HT22 cells were pretreated with/without NAC (5 mM) 2 h before exposure to 8 μg/mL AgNPs for 24 h. (A) Immunofluorescence of Drp1in HT22 cells; (B–C) Western blot for protein expression of Drp1, p-Drp1, Fis1, Mfn1, Mfn2, OPA1 in HT22 cells; (D–E) Apoptosis rate of HT22 cells; (F–G) Western blot for protein expression of Bax, Bcl-2, Caspase-3, Cyt-C in HT22 cells. Data are expressed as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 when compared with the control. ^#p < 0.05, ^##p < 0.01 when compared with the AgNPs (8 μg/mL) group.