REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD45 - Pacific Orange; HI30 (1:50)	Invitrogen	RRID:AB_10376143; Cat #MHCD4530
CD14 - PerCP; HCD14 (1:50)	BioLegend	RRID:AB_2564058; Cat #301847
CD56 - PE; HCD56 (1:50)	BioLegend	RRID:AB_604093; Cat #318305
CD3 - PerCP; UCHT1 (1:50)	BioLegend	RRID:AB_893300; Cat #300427
CD4 - PerCP; SK3 (1:50)	BioLegend	RRID:AB_2563326; Cat #344624
CD8 - Pacific Orange; 3B5 (1:50)	Invitrogen	RRID:AB_10372066; Cat #MHCD0830
CD45RA - Alexa700; HI100 (1:100)	BioLegend	RRID:AB_493762; Cat #304119
CD25 - PE; 2A3 (1:30)	BD Biosciences	RRID:AB_2783790; Cat #341011
PD1 – PE/Cy7; EH12.1 (1:50)	BD Biosciences	RRID:AB_10611585; Cat #561272
TIGIT - APC; MBSA43 (1:20)	eBioscience	RRID:AB_2573305; Cat #17-9500-42
FOXP3 - Pacific Blue; 259D (1:30)	BioLegend	RRID:AB_940354; Cat #320215
HELIOS - Alexa488; 22F6 (1:30)	BioLegend	RRID:AB_10645334; Cat #137213
IFNγ - APC; B27 (1:50)	BioLegend	RRID:AB_315443; Cat #506510
TNFα - Pacific Blue; Mab11 (1:50)	BioLegend	RRID:AB_528965; Cat #502920
CD107a - PerCP/Cy5.5; H4A3 (1:100)	BioLegend	RRID:AB_1227508; Cat #328616
IgG1 - PerCP/Cy5.5; MOPC-21 (1:50)	BioLegend	RRID:AB_893664; Cat #400150
KIR2DL1 – PE/Cy7; Clone HP-MA4 (1:50)	BioLegend	RRID:AB_11415800; Cat #39512
7AAD (1:20)	Thermo Fisher	Cat #A1310
Biological samples
Human placental samples	Local hospital and clinics	N/A
Chemicals, peptides, and recombinant proteins
Percoll	GE Healthcare	Cat #17-5445-02
DNase I	Sigma	Cat #DN25
Collagenase IV	Sigma-Aldrich	Cat #C5138
Recombinant human EGF	PeproTech	Cat #AF-100-15
Human gonadotropic hormone (HcG)	Sigma-Aldrich	Cat #C1063
Ionomycin	Sigma	Cat #I9657
Phorbol 12-myristate 13-acetate (PMA)	Sigma-Aldrich	Cat #P1585
New born calf serum	GIBCO	Cat #16010159
Human AB serum	Corning	Cat #35060CI
Fetal bovine serum (FBS)	Seradigm	Cat #1500-500
LPS	Sigma-Aldrich	Cat #916374
Selenium, transferrin, 100×	Gibco	Cat #41400-045
10 × PBS	Fisher	Cat #BP399-4
Penicillin and streptomycin	Gibco	Cat #15140-122
RPMI 1640	Gibco	Cat #11875-093
X-vivo Medium	Lonza	Cat #04-380Q
DMEM/F12	Gibco	Cat #11320-033
Human fibronectin	Corning	Cat #354008
Compensation Beads, Anti-Mouse Ig K/ Negative Control Compensation Particles Set	BD Biosciences	Cat #552843
Critical commercial assays
FOXP3 fix and perm kit	Invitrogen	Cat #00-5523-00
Software and algorithms
BD FACSDIVA software	BD Biosciences	http://www.bdbiosciences.com/us/instruments/research/software/flowcytometry-acquisition/bd-facsdivasoftware/m/111112/features
FlowJo software	FlowJo	https://www.flowjo.com/
Other
Cell dissociation sieve	Millipore Sigma	CD1-1KT (mesh size 60)
Cell strainers 40 μm, 70 μm, 100 μm	Thermo Fisher	Cat #22363547; Cat #22363548; Cat #22363549
15 mL Centrifuge tubes	Thermo Fisher	Cat #14-955-238
50 mL Conical centrifuge tubes	Thermo Fisher	Cat #14-955-240
Curved tip tweezers
6 ½” scissors
Cell sorter	BD Aria/Sony Nano/ Bigfoot
Flow cytometer analyzer	BD Fortessa/Cytek Aurora
−20°C freezer
−80°C freezer
4°C fridge
Liquid nitrogen storage tank
BSL2 biosafety cabinet
Shaking water bath 37°C
Centrifuge
37°C 5% CO2 Incubator