Cell culture Medium B

Reagent	Final concentration	Amount
DMEM/F12		44 mL
Insulin-Transferrin-Selenium, Ethanolamine, 100× (ITS-X)	1×	0.5 mL
Penicillin and Streptomycin 100×	1×	0.5 mL
Human chorionic gonadotropin (HcG) (2.5 × 106 units/mL)	400 units/mL	8 μL
Epidermal growth Factor (EGF) (100 μg/mL)	5 ng/mL	2.5 μL
New born Calf serum	10%	5 mL
Total		50 mL

Should be made fresh on the day of use.