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. 2023 May 20;8:72. doi: 10.1038/s41541-023-00675-1

Fig. 1. Generation and purification of recombinant fusion proteins.

Fig. 1

a Schematic representation of 66NC, 66CN, 90NC, and 90CN constructs. b Purification of a recombinant 90CN fusion protein. line M, protein molecular weight marker; line 1, 90CN protein after renaturation; line 2, flow-through from the resin; line 3, protein-bound resin before washing; line 4, protein-bound resin after washing; line 5, purified 90CN protein eluted by 500 mM imidazole. c Purification of a recombinant 90NC fusion protein. line M, protein molecular weight marker; line 1, 90NC protein after renaturation; line 2, flow-through from the resin; line 3, protein-bound resin before washing; line 4, protein-bound resin after washing; line 5, purified 90NC protein eluted by 500 mM imidazole. d Purification of the recombinant 66CN and 66NC fusion protein. line M, protein molecular weight marker; line 1, flow-through from the resin; line 2, protein-bound resin before washing; line 3, protein-bound resin after washing; line 4 and 5, purified 66CN protein eluted by 500 mM and 1 M imidazole, respectively; line 6, protein-bound resin after elution; line 7, flow-through from the resin; line 8, protein-bound resin before washing; line 9, protein-bound resin after washing; line 10 and 11, purified 66NC protein eluted by 500 mM and 1 M imidazole, respectively. e Purification of a recombinant 74 F fusion protein. line M, protein molecular weight marker; line 1, flow-through from the resin; line 2, protein-bound resin before washing; line 3, protein-bound resin after washing; line 4 and 5, purified 74 F protein eluted by 200 mM and 500 mM imidazole, respectively. All gels derive from the same experiment and they were processed in parallel.