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. 2023 May 20;8:72. doi: 10.1038/s41541-023-00675-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Histopathology was evaluated in the liver and intestine sections stained with hematoxylin and eosin from mice challenged by intraperitoneal injection with MAP K-10 for 2 weeks after vaccination. An image represents one experiment with at least five independent replicates. a Histopathological changes in the liver (scale bars, 200 μm (top) and 50 μm (bottom)). Montanide ISA 61 VG (61 VG): macro-granulomas (green arrow) and multiple microgranulomas (black arrow), 66NC: multiple microgranulomas (black arrow), 74 F: macro-granulomas (green arrow) and multiple microgranulomas (black arrow). The bottom images are enlarged from the outlined areas of the top images. b Liver histopathology score. c Histopathological changes in the intestine (scale bars, 200 μm (top) and 50 μm (bottom)). Montanide ISA 61 VG (61 VG): macro-granulomas (green arrow) and inflammatory cell infiltration (blue arrow), 66NC: without pathologies, 74 F: macro-granulomas (green arrow) and inflammatory cell infiltration (blue arrow). The bottom images are enlarged from the outlined areas of the top images. d Intestine histopathology score. b, d Mean ± SEM and one-way ANOVA were used to analyze statistical significance followed by Tukey’s multiple comparisons tests. ns, non-significant; *P < 0.05; **P < 0.01.