TABLE 3.
Effect of CoA addition on the rate of α-ketoglutarate decarboxylationa
| Crude extracts | α-KG decarboxylation (nmol min−1 mg of protein−1)
|
|
|---|---|---|
| Without CoA | With CoA | |
| wt, cultured | 1.6 | 5.7 |
| sucA, cultured | 1.5 | 1.6 |
| wt, bacteroids | 3.3 | 6.4 |
| sucA, bacteroids | 3.2 | 3.4 |
a
Crude extracts were prepared from wild type (wt) or sucA mutant (sucA) cells cultured on malate or isolated from nodules (bacteroids). Extracts were assayed for α-ketoglutarate (α-KG) decarboxylation with or without CoA. Assay mixtures contained 1 mM α-ketoglutarate (0.1 μCi), 0.12 to 0.3 mg of protein, thiamine pyrophosphate, and NAD. Assays were terminated 10 min after substrate addition. All values are from single determinations.