TABLE 5.
Activities of α-ketoglutarate-catabolizing enzymes in crude extracts from various rhizobial strainsa
| Strain | Sp act (nmol min−1 mg of protein−1) of:
|
|||
|---|---|---|---|---|
| KGDH | KDC | SSDH (NAD) | SSDH (NADP) | |
| M. loti R7A | 22.7 | 86.9 | 45.2 | 109.8 |
| R. leguminosarum 3841 (wild type) | 91.6 | 2.4 | 12.4 | 6.0 |
| R. leguminosarum RU156 (sucA mutant) | 0 | 5.6 | 10.8 | 4.4 |
a
Cells were grown on minimal medium with malate for a carbon source. Crude extracts were prepared and assayed as described in Materials and Methods. All values are from single determinations. Abbreviations: KGDH, α-ketoglutarate dehydrogenase; KDC, α-ketoglutarate decarboxylase; SSDH, succinic semialdehyde dehydrogenase.