REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD45-PE (Clone: 30-F11) (1 μg injected per mouse, working concentration of 10 μg/mL)	BioLegend	Cat# 103106; RRID: AB_312971
CD45-APC (Clone: 30-F11) (0.05 μg used per stained sample)	BioLegend	Cat# 103112; RRID: AB_312977
CD138-BV421 (Clone: 281-2) (0.02 μg used per stained sample)	BD Biosciences	Cat# 562610; RRID: AB_11153126
IgD-BV605 (Clone: 11-26c.2a) (0.04 μg used per stained sample)	BioLegend	Cat# 405727; RRID: AB_2562887
CD90.2-BV605 (Clone: 53-2.1) (0.04 μg used per stained sample)	BD Biosciences	Cat# 563008; RRID: AB_2665477
CD45R(B220)-PerCP/Cy5.5 (Clone: RA3-6B2) (0.20 μg used per stained sample)	BD Biosciences	Cat# 552771; RRID: AB_394457
CD19-BUV395 (Clone: 1D3) (0.60 μg used per stained sample)	BD Biosciences	Cat# 563557; RRID: AB_2722495
CD16/32-Unlabeled (Clone: 93) (Fc receptor blocking) (1.00 μg used per stained sample)	Thermo Fisher Scientific	Cat# 14-0161-86; RRID: AB_467135
Chemicals, peptides, and recombinant proteins
Bovine serum albumin (DNase- and protease-free)	Fisher Scientific	Cat# BP9706100
Dulbecco’s phosphate buffered saline powder (calcium and magnesium free)	Gibco	Cat# 21600-010
AErrane (isoflurane) 100% liquid	Baxter	Cat# CA2L9108
NH4Cl	Fisher Scientific	Cat# A661-500
KHCO3	Fisher Scientific	Cat# P235-500
Na2-EDTA	Fisher Scientific	Cat# BP120-1
Trypan Blue powder	Fisher Scientific	Cat# AC189350250
Experimental models: Organisms/strains
Mouse: B6.Cg-Tg(Prdm1-EYFP)1Mnz/J (female and male mice heterozygous for the Prdm1-eYFP transgene were used at 3 months of age)	The Jackson Laboratory	JAX: 008828; RRID: IMSR_JAX:008828
Software and algorithms
FlowJo (v10)	BD Biosciences	RRID: SCR_008520
Other
Frosted microscope slides	Fisher Scientific	Cat# 22-034-486
BD lo-dose U-100 insulin syringes w/ 28G needle	Fisher Scientific	Cat# 14-826-79
60-mm sterile petri dishes	Fisher Scientific	Cat# FB0875713A
BD vacutainer collection tubes	Fisher Scientific	Cat# 02-683-45
5-mL polystyrene test tubes	Fisher Scientific	Cat# FB1496110
5-mL polystyrene test tubes with cell strainer snap cap	Fisher Scientific	Cat# 08-771-23
5-mL BD syringes with Luer-Lok	Fisher Scientific	Cat# 14-829-45
BD hypodermic needles (23G)	Fisher Scientific	Cat# 14-826-6B
40-μm sterile cell strainers	Fisher Scientific	Cat# 22-363-547
Veterinary anesthesia machine	Dispomed	https://www.dispomed.com/product-category/veterinary-anesthesia/veterinary-anesthesia-machines/
BD LSRFortessa flow cytometer	BD Biosciences	https://www.bdbiosciences.com/en-ca/products/instruments/flow-cytometers/research-cell-analyzers/bd-lsrfortessa
Sorvall X4R Pro-MD refrigerated benchtop centrifuge	Thermo Fisher Scientific	Cat# 75009521