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. Author manuscript; available in PMC: 2023 May 20.
Published in final edited form as: Chem Rev. 2022 Jul 11;122(14):11974–12045. doi: 10.1021/acs.chemrev.2c00106

Figure 20.

Figure 20.

Crystal structures of target proteins for AlaBpy incorporation. (a) Catabolite activator protein (CAP) with the Lys26 target location of AlaBpy incorporation highlighted (PDB ID: 1J59).280,284 (b) Transcription factor protein Zif268 with target residues for randomization highlighted. Native metal binding residues are highlighted in light gray, while additional target residues are highlighted in blue (PDB ID: 1ZAA).288,285 (c) LmrR with target residues for AlaBpy incorporation highlighted (d) in light gray, with additional SCS target residues highlighted in light blue (PDB ID: 3F8B).286,287 (e) Designed AlaBpy-incorporated protein scaffold based on indole-3-glycerolphosphate synthase containing bound Ni2+ (PDB ID: 4IXO) and (f) Co2+ (PDB ID: 4IWW). PCS residues are highlighted in light gray, while SCS target residues are highlighted in light blue.289 Atom coloring: N (blue); O (red); S (yellow); Fe (orange); Co (salmon); Ni (green).