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. Author manuscript; available in PMC: 2023 May 20.
Published in final edited form as: Chem Rev. 2022 Jul 11;122(14):11974–12045. doi: 10.1021/acs.chemrev.2c00106

Figure 21.

Figure 21.

Crystal structures of target proteins for Cys-maleimide linkages. (a) β-Lactoglobulin isoform B (β-LG) with the target Cys121 for thiosuccinimide formation highlighted in light gray (PDB ID: 4IB9).296,297 (b) Steroid carrier protein SL (SCP-2L) containing an Ala100Cys mutation as the target for thiosuccinimide formation (highlighted in light gray). An additional target residue, Phe94, targeted for SCS modifications, is highlighted in light blue (PDB ID: 6Z1W).298,299 (c) Xylanase A (Xln) with target residue for Ser212 (for the Ser212Cys incorporation highlighted in light gray, with additional SCS target residues highlighted in light blue) (PDB ID: 1E0W).300,301 Atom coloring: O (red); S (yellow).