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(a) CuA site of TtCuA. (b) Original heme pocket of CcP. The three residues Trp51, His52, and Arg48 were engineered as the PCS core for CuA. (c) De novo designed CuA protein and (d) CuA structure. Reprinted with permission from references 445 and 446. Copyright 2012 and 2020 American Chemical Society.
