Skip to main content
. Author manuscript; available in PMC: 2023 May 20.
Published in final edited form as: Chem Rev. 2022 Jul 11;122(14):11974–12045. doi: 10.1021/acs.chemrev.2c00106

Figure 43.

Figure 43.

(a) LmrR variant catalyzing the Michael addition reaction with an aniline moiety on an unnatural amino acid residue to activate the ketone Michael acceptor. The structure was modeled from PDB ID 6R1L,492 and the scheme was adapted from ref 464. (b) Two cupin variants with different enantioselectivities of the Michael addition reaction. The insets at the bottom demonstrate how the hydrogen bond with Cys106 changes the orientation of the substrate and thereby alters the product enantioselectivity. Reprinted with permission from refs 495 and 496. Copyright 2020 Nature Publishing Group and Wiley-VCH.