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(a) Structures of G65Y-CuBMb(+6), showing the engineered Lys in blue, and cyt b5 (PDB ID: 1CYO).526 (b) Oxidase activity of G65Y-CuBMb(+6) in comparison with that of native cyt cbb3 oxidase. The black arrows indicate the addition of reductant, and the double arrow shows the injection of native cyt cbb3 oxidase. Reprinted with permission from ref 525. Copyright 2015 American Chemical Society.
