Figure 3.

ZNF593-AS interrupts IRF3-dependent signaling via suppressing IRF3 phosphorylation

(A) Flow cytometry analysis showed ZNF593-AS knockdown accelerated PA-induced apoptosis of HL-1 cells. (B) Flow cytometry analysis showed ZNF593-AS overexpression antagonized PA-induced apoptosis of HL-1 cells. (C) Scatterplot diagram of DEGs between control and ZNF593-AS overexpression groups in PA-treated HL-1 cells. Blue points represent downregulated genes, red points represent upregulated genes, gray points represent non-DEGs, and black points represent Irf3 (fold change >2 or <0.5, p < 0.05). (D) RT-qPCR validation of the expression of representative IRF3-dependent genes. (E) Western blotting analysis of phosphorylated and total IRF3 in HL-1 lysates of control and ZNF593-AS overexpression under PA treatment. Student’s t test was used for comparisons in (D) and (E). One-way ANOVA with the Tukey post-test was used in (A) and (B).