FIG. 5.

Binding of d-[¹⁴C]alanyl–Dcp and d-[¹⁴C]alanyl–ACP (A) and ligation of d-[¹⁴C]alanine to Dcp and ACP (B) in the presence of ΔDltD on NC membranes. (A) Either d-[¹⁴C]alanyl–Dcp or d-[¹⁴C]alanyl–ACP was incubated with the NC membrane containing increasing amounts of ΔDltD, and the amount bound was quantified as described in Materials and Methods. (B) Either Dcp or ACP was incubated with the membrane containing ΔDltD, and after washing, the filter was incubated in the presence of Dcl, ATP, and d-[¹⁴C]alanine as described in Materials and Methods. b, 8 μg of ΔDltD previously treated at 100°C for 2 min.