FIG. 7.

Time courses of d-[¹⁴C]alanyl–ACP hydrolysis in the absence of membranes (■) and in the presence of DltD⁺(□), DltD⁻(▴), and DltD⁻/ DltD⁺(▵) membranes. The 250-μl reaction mixture contained 6.5 nmol of d-[¹⁴C]alanyl–ACP and 130 μg of membranes (except for the control) in 10 mM bis-Tris and 30 mM MgCl₂. Samples (50 μl) were removed from the incubation mixture (37°C) at the indicated times, and the amounts of d-[¹⁴C]alanyl–ACP remaining were measured by precipitation with 10% trichloroacetic acid according to the method of Heaton and Neuhaus (19).