Fig. 8.

Intravitreally injected α-synuclein causes the cortical inflammation 5 months after injection. A Representative images of microglia (Iba-1–positive cells) in the visual cortex, perirhinal cortex, and entorhinal cortex. B–D Quantification of the optical density of Iba-1 immunostaining in the cortical areas. E–G Microglial density in the visual, perirhinal and entorhinal cortices, normalized per area and expressed as microglia cell number per 10,000 µm². (H) Representative images of TNF-α immunostaining in the cortical areas. (I-K) Quantification of TNF-α–positive cell counts in the cortical areas per 10,000 µm². Scale bar: 100 µm. Data are expressed as means ± s.e.m. (PBS, n = 10; PFF, n = 10; *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t-test)