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. 2023 Apr 19;24(3):bbad133. doi: 10.1093/bib/bbad133

Figure 2.

Figure 2

Visualization of the interactions between PAM nucleotides and PAM-recognizing amino acids. (A) 4UN3 (SpCas9) with TGG PAM; (B) 5F9R (SpCas9) with TGG PAM; (C) 5AXW (SaCas9) with TTGGGT PAM; (D) 5CZZ (SaCas9) with TTGAAT PAM; (E) 6M0W (St1Cas9) with AAAGAA PAM; (F) 5B2O (FnCas9) with TGG PAM; (G) 6JDV (Nme1Cas9) with ATATGATT PAM; (H) 6JE3 (Nme2Cas9) with AGGCCC PAM; (I) 6JOO (CdCas9) with GGGTAAT PAM; (J) 6WBR (AceCas9) with ATACC PAM; (K) 5B43 (AsCas12a) with TTTA PAM and (L) 5XUS (LbCas12a) with TTTA PAM. Nucleotides on nontarget and target strands are shown as yellow and magenta sticks, respectively; nontarget-stranded nucleotides are marked with asterisks. Amino acids are shown in green sticks. Hydrogen bonds are shown as green dashed lines.