Skip to main content
. 2023 Apr 19;24(3):bbad133. doi: 10.1093/bib/bbad133

Figure 5.

Figure 5

UniDesign predicted PAM-interacting amino acids based on consensus PAM-bearing Cas protein variants. (A) 4UN3 (SpCas9) with four consensus PAM variants; (B) 5F9R (SpCas9) with four consensus PAM variants; (C) 5AXW (SaCas9) with 64 consensus PAM variants; (D) 5CZZ (SaCas9) with 64 consensus PAM variants; (E) 6M0W (St1Cas9) with 32 consensus PAM variants; (F) 5B2O (FnCas9) with four consensus PAM variants; (G) 6JDV (Nme1Cas9) with 16 consensus PAM variants; (H) 6JE3 (Nme2Cas9) with 256 consensus PAM variants; (I) 6JOO (CdCas9) with 432 consensus PAM variants; (J) 6WBR (AceCas9) with 64 consensus PAM variants; (K) 5B43 (AsCas12a) with three consensus PAM variants and (L) 5XUS (LbCas12a) with three consensus PAM variants. Note that 16 and 432 consensus PAM variants were obtained for Nme1Cas9 and CdCas9 because their first two or one PAM positions were not varied, respectively.